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Fig. 3. Mean normalized spectral sensitivity of rainbow trout (±1 standard error) using electroretinogram (ERG) recording. For each treatment condition, panels on the left side show the spectral sensitivity curves for the control and treatment conditions and the panels on the right side show the difference curves between the control and treatment spectral sensitivity. Green points represent the control and red points represent the treatment condition. The sensitivity measurements were normalized to 360 nm. (A) Mean normalized spectral sensitivity of control fish where the spectral sensitivity was measured using a broad-spectrum tungsten halogen background (N=3), and treatment fish where spectral sensitivity was measured using a broad spectrum and UG-11 background (N=3). (B) Mean difference spectrum as a result of short wavelength chromatic adaptation (UG-11 filter). The black line fitted to the data points at the shortwave end of the spectrum represents an additive function based on the UVS and short wavelength-sensitive (SWS) visual pigment templates. (C) Mean normalized spectral sensitivity of the same control fish as in A, and treatment fish where spectral sensitivity was measured using a broad spectrum and 600 nm long pass background (N=3). (D) Mean difference spectrum as a result of long wavelength chromatic adaptation (600 nm long pass filter). The black line fitted to the points represents the LWS cone visual pigment template.

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