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Fig. 4. Relative expression of CS mRNA and of the different COX subunit mRNAs in
hepatocytes of cold- (white bars) and warm- (grey bars) acclimated eelpout
(Z. viviparus). Relative amounts of CS (A–C), COX2 (D–F)
and COX4 (G–I) mRNA were determined in freshly isolated hepatocytes
(A,D,G; N=4) and in cells after incubation at either 4 or 11°C
(B,C,E,F,H,I; N=5–8) under control conditions (white bars) or
with the addition of adenosine (hatched bars). mRNA levels in equal amounts of
total RNA were determined by RPA and normalized to the mean of freshly
isolated hepatocytes from warm-acclimated fish. *Significant
difference from control group incubated at the same temperature;
significant difference from corresponding group incubated
at 4°C. Values are means ± s.e.m.
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