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Figure 4


Fig. 4. Vma2pArch-containing yeast show increased sensitivity to cycloheximide and wortmannin. VMA2{Delta} yeast cells carrying no plasmid (Null) or transformed with yeast B subunit (WT), Vma2pPhe (Phe) or Vma2pArch (Arch) plasmids were grown to log phase, carefully counted so that each type of cell was at the same starting concentration, subjected to serial 10-fold dilutions, and then transferred to plates with control media (A) or media containing sub-lethal doses of various drugs (B) as indicated in Materials and methods for 3 days. Ct, camptothecin; Hu, hydroxyurea; Cx, cycloheximide; Wt, wortmannin; Sm, sulfometuron methyl; Fk, FK506.





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