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Figure 2


Fig. 2. Mutations in the profilin-like motif of yeast B subunit reduce or eliminate actin-binding activity. (A) Alterations in the profilin-like motif made and analyzed in the current study. Amino acids 35–45 in wild-type Vma2p, Vma2pPhe and Vma2pArch are compared. (B) Haine's plot of Vma2pPhe-MBP binding to F-actin. (C) Vma2pArch-MBP does not bind F-actin: Lane 1, supernatant and lane 2 pellet of 4.0 µmol l–1 F-actin and 1.0 µmol l–1 Vma2pArch-MBP. Lanes 3 and 4 are the supernatant and pellet of 8.0 µmol l–1 F-actin and 1.0 µmol l–1 Vma2pArch-MBP supernatant.





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