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Fig. 6. Glucose (A), glycogen (B) and lactate (C) levels, and glucokinase (D) and
lactate dehydrogenase reductase (E) activities in hypothalamus of rainbow
trout incubated in vitro for 1 h at 15°C in modified Hanks'
medium containing 2, 4 or 8 mmol l–1 L-(+)-lactate
alone (control) or with 50 mmol l–1 sodium oxamate, 1 mmol
l–1 4,4'-diisothiocyanatostilbene-2,2'-disulfonic
acid disodium salt hydrate (DIDS), 1 mmol l–1 sodium
dichloroacetate (DCA), 10 mmol l–1
2-deoxy-D-glucose (2-DG), 1 mmol l–1
-cyano-4-hydroxy cinnamate (4-CIN) or 10 mmol l–1
D-glucose. Each value is the mean ± s.e.m. of ten (control)
or three (treatments) independent experiments carried out with pools of
tissues from three to four different fish (21 pools per experiment: seven
treatments times three glucose concentrations). Different letters indicate
significant differences (P<0.05) among treatments within each
lactate concentration. #Significantly different from groups
incubated with 2 mmol l–1 lactate within the same treatment
(P<0.05); 
significantly different from groups
incubated with 4 mmol l–1 lactate within the same treatment
(P<0.05).
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