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Figure 4


Fig. 4. H+-ATPase B subunit expression determined by immunoblotting in ammocoetes (white bars) and transformers (grey bars) of P. marinus. (A) Ammocoetes and transformers (stages 3–5) acclimated to freshwater (FW) and 25{per thousand} salinity and sampled from the Fort River, MA, USA (hatched bars). (B) Ammocoetes and transformers (stage >=6) acclimated to deionized water (DW), FW or a salinity of 10, 20, 30 and 35{per thousand}. Bars with like characters are not significantly different (ANOVA; P<0.05). Upper and lower case characters are used for transformers (TR) and ammocoetes (AM), respectively, in B. Representative immunoblots of sea lamprey ammocoete and transformer gill probed with the H+-ATPase B subunit antibody B2/BvA1 are shown above their respective bars.





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