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Fig. 4. Max projection images at higher magnification than in Fig. 3 of regions of the alimentary canal showing the location of transport proteins. (A) Posterior cap cells and other scattered cells (white arrows) in the gastric caeca are labeled intensely with the H⁺ V-ATPase antibody (red) whereas the rest of the caecal cells are stained with Na⁺/K⁺ P-ATPase antibody (green). (B) The DAR cells are labeled with H⁺ V-ATPase antibody (red). (C) The rest of the rectum is labeled with Na⁺/K⁺ P-ATPase antibody (green). (D) H⁺ V-ATPase (red) and Na⁺/K⁺ P-ATPase (green) are colocalized in the rectum. (E) AgNHA1 (red) and Na⁺/K⁺ P-ATPase (green) are also colocalized in the rectum; the DAR cells have a greenish background. (F,H) Na⁺/K⁺ P-ATPase is present on the dorsal side of the rectum with the conspicuous absence of staining in the DAR cells. (G) In a ventral view of the rectum, Na⁺/K⁺ P-ATPase labeling (green) is widespread. (A,D,E) The yellow color results from the colocalization of AgNHA1 or H⁺ V-ATPase (both red) with Na⁺/K⁺ P-ATPase (green). (I) H⁺ V-ATPase is present on the apical membranes of the principal cells in the Malpighian tubules (MT). (J,K) AgNHA1 and the Na⁺/K⁺ P-ATPase (green) are present in the entire nervous system. Shown here are the trilobed thoracic ganglion (TTG; J) and abdominal ganglion (AG; K). Scale bars in A and B (for B–H), 150 µm; in I (for I–K), 100 µm.

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