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Fig. 5. The relative mRNA levels of DNMT1, DNMT3, MBD2, MeCP2 in monocytes cultured
for different times (A) and with different antagonists (B). Fluorescence was
versus the PCR cycle number for both reactions and each sample
dilubition is indicated. Each gene RNA level was acquired from the value of
the threshold cycle (Ct) of the real-time PCR relative to that of GAPDH using
Eqn 1. The final results,
expressed as N-fold differences in target gene expression relative to the
calibrator, termed `Ntarget', are determined as following:
N=2
Ct(sample)–Ct(calibrator)
(Eqn 2), where Ct values
of the calibrator and sample were determined by subtracting the Ct value of
the target gene from the Ct value. Values are means ± s.e.m.,
representative of three separate experiments.
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