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Figure 4


Fig. 4. Relative EC-SOD protein (A) and mRNA (B) levels. Monocytes were treated with 100 µmol l–1 Hcy in control medium for 24, 48 or 72 h. Total RNA was isolated and real-time PCR was carried out to detect mRNA levels. Protein levels of EC-SOD (A) were examined by western blotting (B). Values are means ± s.e.m. from 3 separate experiments with triplicated samples (N=6).*P<0.05, compared with positive group; §P<0.05, compared with 100 µmol l–1 Hcy group (72 h).





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