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Fig. 1. Sequences of EC-SOD primers and probes. The probes for modified EC-SOD are
indicated by shaded boxes, and the polymerase chain reaction (PCR) primers for
the EC-SOD sequence are indicated by arrows and bold, italic type. The
sequence of EC-SOD after sodium bisulfite modification is also shown. The
solid line arrows indicate the extension direction. Methylated and
unmethylated PCR have the same primers. Fluorescence detection was carried out
by two passages. When PCR was carried out with methylation-modified gDNA, the
fluorescence of the methylated probe was detected by the one passage, similar
to PCR with unmethylation-modified gDNA. The other kind of fluorescence of
unmethylated probes was detected by the other passages. Upper row, original
sequence; lower row, bisulfite modified sequence (for display, assume all CpG
sites are methylated); +, CpG site; non CpG `C' converted to `T';
>>>, left primer; <<<, right primer.
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