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Figure 5


Fig. 5. In situ and cultured neurons display different cellular localization of 5-HT1Hel and 5-HT7Hel receptors. In situ, 5-HT1Hel-like immunoreactivity (A) and 5-HT7Hel-like immunoreactivity (B) was restricted to neurites in neuropile (filled arrows) and neurite tracts within connectives and nerves (arrowhead; also see Fig. 4). Cell bodies (open arrows) were always unstained as revealed by overlaying the differential interference contrast pattern on the fluorescence signal. In contrast, after isolation and culture of cerebral neuron C1, 5-HT1Hel-like immunoreactivity occurred primarily in the cell body (C and D). Preabsorption of antibody with Keyhole limpet hemocyanin (D) and replacement of antiserum with pre-immune serum (E) provided positive and negative controls for antibody specificity, respectively. Scale bar, 50 µm.





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