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Figure 2


Fig. 2. cGMP is transported by tubules. (A) Effect of depolarisation on the cGMP transport ratio. Leucokinin (100 µmol l–1) was used to depolarise the Malpighian tubule 30 min prior to the addition of cGMP (100 µmol l–1; values are means ± s.e.m.; N>=10; ***P<0.0001, Student's t-test). (B) cGMP-dependent kinase (cGK) activity in tubules is stimulated by fluid secreted from cGMP-treated Malpighian tubules. Data are expressed as mean (± s.e.m.) cGK activity, in pmol ATP min–1 mg–1 protein (N=3). cGK activity in tubule extracts is significantly stimulated by secreted drops from cGMP-treated tubules or by 100 µmol l–1 cGMP, but not by secreted fluid from non-treated tubules (***P<0.0001, Student's t-test).





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