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Fig. 3. Helisoma buccal neuron B27 is inhibited by glutamate and excited
by KA. (A) Diagram of the buccal ganglia showing the bilateral location of
left and right (shaded in grey) B27 neuron cell bodies (adapted from
Quinlan et al., 1995). (B) A
left B27 neuron was injected with Lucifer Yellow. Its main axons extend out
through the ipsilateral and contralateral LBN. Fine processes extending from
the cell body and axons are partially occluded by the brightness of the cell
body staining. The fluorescence image is a composite of 17 separate images and
is layered on a DIC image of the same field (see Materials and methods). Scale
bar, 100 µm. (C) Glutamate dose-dependently reduced AP frequency in a B27
neuron. Membrane potential was measured using standard intracellular recording
techniques. Drugs were perfused during the time indicated by the horizontal
bars. Breaks in the recording reflect 1–2 min of saline perfusion.
Arrowhead, 0 mV. (D) Dose-dependent reduction by glutamate of mean
(±s.e.m.) AP frequency of eight cells (*P<0.01).
(E) Mean (±s.e.m.) AP frequency of B27 neurons was increased during
perfusion of 30 µmol l–1 KA
(#P=0.0002, N=10), but not 10 µmol
l–1 KA (N=9). (F) CNQX (50 µmol
l–1) had no effect on KA-induced excitation of some B27
neurons (upper trace), and inhibited excitation in others (lower trace). Drugs
were perfused during the time indicated by the horizontal lines. Arrowheads
indicate 0 mV. (G) CNQX reduced the KA-induced increase in mean
(±s.e.m.) AP frequency in B27 neurons (+P<0.05,
N=9).
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