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Fig. 2. Helisoma buccal neuron B19 is inhibited by glutamate and excited
by KA. (A) Diagram of the buccal ganglia showing the bilateral location of
left and right (shaded in grey) B19 neuron cell bodies (adapted from
Quinlan et al., 1995). (B) A
right B19 neuron was injected with Lucifer Yellow. Its main projections extend
ipsilaterally out through the right VBN and LBN, and contralaterally out
through the left VBN. Extensive neurites leave the cell body and axonal
projections; some are obscured by the bright cell body staining. Fluorescence
image is a composite of 31 separate images and is layered on an HMC image of
the same field (see Materials and methods). Scale bar, 100 µm. (C)
Glutamate, applied for the durations indicated by horizontal bars,
dose-dependently reduced AP frequency in a B19 neuron. Breaks in the trace
represent 2–3 min of saline perfusion. Arrowhead indicates 0 mV. (D)
Glutamate dose-dependently reduced mean (±s.e.m.) AP frequency of B19
neurons (*P<0.05, N=9). (E) KA had no effect
on mean (±s.e.m.) AP frequency at 10 µmol l–1
(N=9), but significantly increased frequency at 30 µmol
l–1 (+P<0.03; N=13). (F) CNQX
had no effect on mean (±s.e.m.) AP frequency in B19 neurons
(N=9).
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