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Figure 4


Fig. 4. Microfluorometric measurements of oxygen content in the lumen of isolated salivary gland tubules injected with polystyrene beads containing the O2-sensitive luminescent dye PtPFPP. 100% O2 concentration in A–C corresponds to the O2 content in the bath PS, which is in equilibrium with ambient air. (A) 10 nmol l–1 5-HT stimulates cellular respiration and a drop in luminal O2 concentration that is not significantly reduced in the presence of concanamycin A (A,D). The 8-CPT-cAMP-induced drop in luminal O2 concentration is significantly reduced by concanamycin A (B,D). Application of 1 µmol l–1 thapsigargin in Ca2+-free PS causes an increase in luminal O2 concentration, and the 5-HT-induced drop in luminal O2 concentration is significantly reduced under these conditions (C,D). Data in D are means ± s.e.m.; the number of experiments is given in parentheses; *P<0.05.





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