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Figure 5


Fig. 5. Micrographs of cross sections of the tegument of the yellow spider. (A) Unstained section of the light-yellow spider, showing the presence of two types of granules: type II (short, thick arrow) and type III (long, thin arrow) (bar, 10 µm). (B) Same region as in A observed under UV light. The arrow indicates the fluorescent type II granules. Note the absence of fluorescence stemming from the isolated type III granules (bar, 10 µm). (C) Electron micrograph of the same region as A and B showing in detail the granules type II and type III (ommochromes) (bar, 2 µm). The inset shows a detail of a Golgi body (arrow) (bar, 0.5 µm). (D) Unstained section of the bright-yellow spider, with the epidermal cells full of type III granules (arrow) (bar, 20 µm). (E) Electron micrograph of the same region as D. Note the high concentration of the homogeneous granules type III (arrow) filling the cell (bar, 10 µm). The inset shows a detail of the type III granules (bar, 1 µm). (F) Detail of the epidermal cell cytoplasm showing the abundance of glycogen rosettes (asterisks) and a Golgi region (arrow) (bar, 0.5 µm). C, cuticle; E, epidermal cell layer; N, nucleus; Gr II, granule type II; Gr III, granule type III.





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