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Figure 4


Fig. 4. Human insulin dose-dependently induced hydrogen peroxide synthesis in A. stephensi MSQ43 cells. MSQ43 cells were stimulated with human insulin (Ins, 0.17–17 µmol l–1) and assayed for H2O2 using DCF-DA. Controls included no treatment (No TX), treatment with an equivalent volume of insulin buffer (Hepes), treatment with 100 units ml–1 catalase (CAT), treatment with 500 µmol l–1 H2O2, and pre-treatment with catalase (+CAT) followed by treatment with H2O2 or insulin. Data are represented as mean relative fluorescence units ± s.e.m. (N=3). An asterisk denotes a significant difference ({alpha}=0.05) between treatment and no treatment (No TX).





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