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Figure 3


Fig. 3. Example survivorship curve showing that human insulin provided by artificial bloodmeal increased the mortality of A. stephensi relative to controls and that this effect was reversed by the provision of the cell-permeable SOD mimetic agent MnTBAP in the presence of insulin. This example corresponds to experiment 5, Table 2. Female mosquitoes were provided with 1.7x10–3 µmol l–1 human insulin, 0.05 mmol l–1 MnTBAP alone (not shown), human insulin + MnTBAP, or with an equivalent volume of insulin buffer by artificial bloodmeal every Monday, Wednesday and Friday until all insects were dead. Oviposition cups and 10% sucrose pads were provided between bloodmeals. Dead insects were counted and removed daily from treatment and control cartons.





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