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Fig. 3. S2 cell viability following Cu limitation. S2 cells were pre-treated with
Mvl or control dsRNA for 6 days. Real-time PCR demonstrated
Mvl was suppressed to 47.3±16.1% (mean ± s.e.m.) of
control after 6 days. Cells were exposed to basal medium or Cu-limited medium
using 10 µmol l–1 Diamsar for 6 days and viable cells were
counted. Values are mean ± s.e.m. of nine replicates from three
independent experiments. An independent samples t-test was used to
determine significant differences. *P<0.05 compared to
basal medium, 
P<0.05 compared to control cells
exposed to Cu-limited medium.
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