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Fig. 6. Membrane currents under isotonic and hypotonic conditions. (A) Superimposed
membrane currents upon shifting Em for 1 s from –50
mV to values ranging from –120 to –40 mV (10 mV increments, 5 s
intervals) under isotonic conditions (SLS) and after 5 min in hypotonic
solution (–40 mmol l–1 NaCl). Subtraction of the
current needed under isotonic conditions from the respective current under
hypotonic conditions isolated the swelling-activated membrane current
(`Difference'). The dependence of this current on Em is
shown in B. The traces are means of 34 experiments. Before averaging, the
single traces were filtered through an 8-pole Bessel filter (100 Hz). The
performance of the voltage-clamp protocol took 
1 min; in the meantime,
the recording system was in the current-clamp mode. (B) Voltage dependence of
the averaged swelling-activated membrane current under control conditions (see
A), in Cl––free solution and in the presence of 0.5
mmol l–1 DIDS. Data are means ± s.d. of N=47
(control, included are 13 experiments with a clamp duration of only 0.5 s),
N=7 (Cl– free) and N=11 experiments (DIDS).
Arrowhead indicates the reversal potential of the swelling-activated current
under control conditions.
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