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Fig. 1. Paraffin and cryostat sections of euthyroid and hypothyroid rat Harderian
glands (HGs). (A) The glandular cells of euthyroid rat HG contain dense
basophilic secretory granules and basal nuclei. L, lumen. (B) Glandular cells
from hypothyroid rat HG show pale secretory granules. The acinar lumina (L)
are filled with secretory granules mixed with nuclei and cytoplasmic
fragments. A and B are Mallory stained. (C) The acinar cells from euthyroid
rat HG are positive for the mercury–Bromophenol Blue reaction for
protein. (D) In hypothyroid rat HG some glandular cells are weakly positive
for the mercury–Bromophenol Blue reaction (blue stained), whereas the
others are negative (brown stained). Pycnotic nuclei (arrows) can be observed.
Acini coalescing can also be observed (asterisks). (E) The glandular
epithelium of euthyroid rat HG is weakly positive for the AB/PAS reaction. L,
lumen. (F) Glandular cells from hypothyroid rat HG are positive for AB/PAS
reaction. The lumina (L) are filled with strongly positive AB/PAS secretion.
(G) Cryostat section from euthyroid rat HG. Large Sudan Black-positive
vacuoles (arrowheads) and porphyrin accretions (arrows) are present in
glandular cells. (H) Cryostat section of hypothyroid rat HG. A few small
vacuoles stained with Sudan Black are observed outside the acini (arrows).
Scale bars, 16 µm.
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