spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. G{alpha}s signaling pathway utilized in bioassay. The cyclic AMP-dependent response in oocytes co-expressing the zebrafish cDNA library and CFTR is activated by the binding of a ligand to a membrane receptor, causing the receptor to interact with a G protein (G{alpha}s). Upon dissociation from the heterotrimeric G-protein–receptor complex, the {alpha} subunit activates adenylyl cyclase. This action leads to a cAMP signaling cascade, ending in the activation of CFTR. The output of this cascade is measured by electrophysiology and is the basis of the functional assay.





Right arrow Return to article