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Figure 4


Fig. 4. Effects of various factors on intracellular acidification induced by 1 µmol l–1 DA in duct cells of lobe preparations in CO2/HCO3–-buffered saline. (A) The acidification is still present in CO2/HCO3–-buffered saline (N=5). (B) Bath application of 10 µmol l–1 bumetanide does not influence the acidification (N=5). (C) The combined bath application of 10 µmol l–1 bumetanide and 500 µmol l–1 DIDS strongly reduces the acidification (N=5). (D) The combined bath application of 10 µmol l–1 bumetanide and 50 µmol l–1 EIPA also reduces the acidification dramatically (N=5). (E) In addition, the combined bath application of 10 µmol l–1 bumetanide and 500 µmol l–1 acetozolamide decreases the acidification (N=5).





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