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Figure 3


Fig. 3. Effects of various factors on intracellular acidification induced by 1 µmol l–1 DA in duct cells of lobe preparations in HCO3–-free saline. (A) The acidification is abolished in Cl––free saline (N=4). (B) The acidification is not influenced by bath application of 500 µmol l–1 DIDS (N=9). (C) The acidification is transient following the bath application of 10 µmol l–1 bumetanide (N=6). (D) The combined bath application of 10 µmol l–1 bumetanide and 500 µmol l–1 DIDS abolishes the acidification (N=6). (E) The acidification is not influenced by bath application of 500 µmol l–1 acetozolamide (N=4). (F) The combined bath application of 10 µmol l–1 bumetanide and 500 µmol l–1 acetazolamide reduces the acidification dramatically (N=8).





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