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Figure 6


Fig. 6. Circularized RGD-peptides modulate oxidative burst in the injured nerve. (A–C, left panels) Phase contrast photomicrographs showing the injured RIP nerve cultured in ABS only, 1 µmol l–1 cGRGDSPA and 100 µmol l–1 cGRGDSPA, respectively. Arrows indicate crush zones. (A–C, right panels) Corresponding CM-H2DCF fluorescence signal of the nerves shown in the left panels. (D) Average CM-H2DCF fluorescence intensity of preparations cultured for 1 h in ABS only, ABS + 1 µmol l–1 cGRGDSPA or ABS + 100 µmol l–1 cGRGDSPA. (E) Average CM-H2DCF fluorescence intensity of preparations cultured for 48 h in ABS only, ABS + 1 µmol l–1 cGRGDSPA or ABS + 100 µmol l–1 cGRGDSPA. Note the attenuated CM-H2DCF fluorescence signal in the preparations cultured in the presence of 1 µmol l–1 cGRGDSPA. Image acquisition conditions were exactly the same for all preparations. Scale bar in A–C, 100 µm.





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