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Fig. 5. Reactive oxygen species production depends on the activation status of
endoneurial phagocytes. (Ai and Aii, left panels) DIC images of a spreading
endoneurial phagocyte (i) and non-spreading endoneurial phagocyte (ii). (Ai
and Aii, right panels) Epifluorescence images showing CM-H2DCF
fluorescence (i), or the absence thereof (ii), in the same cells as shown in
the corresponding left panels. Note that the CM-H2DCF fluorescence
is contained inside the activated endoneurial phagocyte. (B) Percentage of
CM-H2DCF fluorescence-positive endoneurial phagocytes when cultured
in ABS only, ABS + 1 µmol l–1 cGRGDSPA and ABS +
100 µmol l–1 cGRGDSPA. Note that independent of
culture condition, the majority of the spreading cells are CM-H2DCF
fluorescence positive while only a very small percentage (<3%) of the
non-spreading cells display CM-H2DCF fluorescence. Image
acquisition conditions were exactly the same for all cells. Scale bar in A, 10
µm.
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