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Fig. 3. Linear RGD-peptides reduce the percentage of phagocytic active cells in
vitro. (Ai) Photograph of an isolated endoneurial phagocyte cultured in
the presence of monodispersed polystyrene carboxylated Fluoresbrite YG
microspheres. (Aii) Photomicrograph showing the large number of fluorescent
microspheres engulfed by the phagocyte shown in Ai. (Bi) Percentage of
spreading cells that internalized uncoated microspheres when cultured in ABS
only, ABS + 100 µmol l–1 SDGRG and ABS + 100 µmol
l–1 GRGDS. (Bii) The distribution of the number of
uncoated microspheres engulfed by spreading cells under the three culture
conditions. Note that the addition of GRGDS had no effect on the
percentage of phagocytic cells engulfing uncoated microspheres nor on the
distribution of the number of engulfed uncoated microspheres. (Ci) Percentage
of spreading cells that phagocytized fibronectin-coated microspheres when
cultured in ABS only, ABS + 100 µmol l–1 SDGRG and ABS +
100 µmol l–1 GRGDS. The addition of GRGDS
significantly reduced the percentage of phagocytic cells. (Cii) Distribution
of the number of engulfed fibronectin-coated microspheres by spreading cells
is shifted to the right (i.e. more microspheres are engulfed) when the cells
were cultured in ABS or ABS + 100 µmol l–1 SDGRG. In
contrast, treatment with GRGDS did not result in a similar increase in
internalization of fibronectin-coated microspheres. Scale bar in A, 10 µm.
***P<0.001.
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