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Figure 4


Fig. 4. Measurement of the gap-junction resistance between principal cells of isolated Malpighian tubules of the yellow fever mosquito Aedes aegypti. (A) The measuring circuit in the tubule modeled as a double cable; (B) reduction of the circuit by eliminating Rsh via the short-circuiting effects of leucokinin-VIII; (C) further reduction of the circuit by combining the parallel resistances in circuit in B. The electromotive forces (E) are neglected in C as they should not be affected by voltage-clamping cell 1. V, R and I have their usual meaning; a, apical membrane; bl, basolateral membrane; lu, tubule lumen; gj, gap junction; nj, non-junction. The non-junctional resistance Rnj includes Ra, Rbl and Rlu.





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