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Fig. 10. RT-PCR analysis of Aedes Malpighian tubules for innexin
transcripts. The image shows PCR products separated on a 1% agarose gel and
stained with ethidium bromide. On the gel are results for PCRs designed to
amplify each Aedes innexin using the primer pairs indicated in
Table 1. For each innexin, the
Malpighian tubule cDNA was used as a template in lane `a', and Malpighian
tubule RNA was used as a template in lane `b'. The lane `mw' is a 1 Kb Plus
DNA Ladder (Invitrogen), in which the first seven bands (starting from the
bottom of gel) correspond to 100, 200, 300, 400, 500, 650 and 850 bp,
respectively.