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Fig. 4. Phylogenies of lepidopteran LW and B opsin genes. The tree is based upon a
neighbor-joining analysis of nucleotide sites using Tamura–Nei model of
evolution with a correction for heterogenous patterns of nucleotide
substitution among lineages. The reliability of the tree was tested using 1000
bootstrap replicates. Only bootstrap support values >50% are shown. (A) The
LW opsin gene tree was reconstructed using the first and second nucleotide
positions (742 sites). (B) The B opsin gene tree was reconstructed using all
nucleotide positions (1055 sites). GenBank accession numbers are as follows:
Agriades glandon (BRh1, DQ402502; BRh2, DQ402503);
Apodemia mormo (BRh, AY587906; LWRh1, AY587907;
LWRh2, AY587908); Bicyclus anynana (Blue, AY918894;
LW, AY918895); Colias philodice (V, AY918899);
Danaus plexippus (Blue, AY605544; LW, AY605545);
Heliconius erato (Blue, AY918906; LW, AY918907);
Heliconius melpomene (Blue, AY918897); Limenitis
arthemis astyanax (Blue, AY918902; LWRh, DQ212962);
Lycaena rubidus (LWRh, AY587901; BRh1, AY587902;
BRh2, AY587903); Manduca sexta (Manop1, L78080;
Manop3, AD001674); Nymphalis antiopa (Blue,
AY918893); Papilio glaucus (PglRh1, AF077189;
PglRh2, AF077190; PglRh3, AF067080; Blue,
AF077192); Papilio xuthus (PxRh1, AB007423; PxRh2,
AB007425; PxRh3, AB007425; PxRh4, AB028217); Pieris
rapae (PrB, AB208675; PrV, AB208674; PrL,
AB188567); Polyommatus icarus (BRh1, DQ402500;
BRh2, DQ402501); Satyrium behrii (BRh1, DQ402498;
BRh2, DQ402499), and Vanessa cardui (VanG,
AY613986; VanB, AY613987). GenBank accession number of the newly
cloned Polyommatus icarus LWRh is EU088114 (orange).
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