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Fig. 6. Identification of the 27 kDa protein species in the total extract obtained
from M. chuni giant basal spicules. (A) An extract from lamellae (la)
of giant basal spicule was obtained and subjected to 10% SDS-PAGE. The 27 kDa
protein is stained with Coomassie Brilliant Blue (lane a). M, size markers.
(B) For the reaction with the labelled inhibitor (biotinylated E-64) the
Monorhaphis spicule proteins were blot transferred and reacted with
biotinylated E-64 as described under Materials and methods. The binding
reaction with this reagent was performed either by direct addition of
biotinylated E-64 to the blot (lane a, –; the 27 kDa protein is
labelled) or after a pretreatment of the blot with a surplus of unlabelled
E-64 (lane b, +).
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