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Figure 5


Fig. 5. Effect of cysteine proteinase inhibitors E-64 and cystatin on the proteolytic activity of cathepsin L (left), as well as spicule extracts from S. domuncula (middle) and M. chuni (right). The activity of the samples was determined either directly (black columns), or following pretreatment with E-64 (dark grey columns) or cystatin (light grey columns). The proteolytic activity was measured with the synthetic substrate Z-Phe-Arg-AMC, and is given as nmol AMC released mg–1 protein min–1. Means (±s.d.) of five independent experiments are given.





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