spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Proteolytic activity of silicatein, present in spicule extract from S. domuncula. Extracts were prepared and separated by electrophoresis. After size separation the gel was incubated overnight at room temperature to identify the zones of proteolytic digestion as outlined under Materials and methods. The migration distance of the cleared zone (24/25 kDa) is characteristic for silicatein (lane a). If the sample from the spicules had been pre-incubated with E-64 (lane b) or with the recombinant sponge cystatin (lane c), no clearance zone could be seen. Form these data we conclude that silicatein, present in the spicule extract, still retains proteolytic activity. M, size markers.





Right arrow Return to article