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Figure 3


Fig. 3. Quantification of relative mRNA abundance of branchial pFXYD. (A) RT-PCR analysis confirmed the primer specificity. M, markers. (B) Comparison between branchial mRNA abundance of freshwater (FW)- and seawater (SW)-acclimated pufferfish, as quantified by real-time PCR. β-Actin was used as an internal control. Values are means ± s.e.m. (N=6). The asterisk indicates a significant difference between the FW and SW groups (P<0.05, Student's t-test).





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