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Fig. 1. Variable N-terminal region of protein isoforms produced by alternative
splicing of the Tnt gene in fall armyworm moths and Glanville
fritillary butterflies. Alternative exons are colored. Traces on the right
show capillary electrophoresis separation of PCR products generated by primers
that hybridize on either side of the alternative exon region and from which we
determined fragment size and relative abundance. Fragment size measurements
match predicted sizes from sequence data. Red peaks are internal size
standards. Inset shows western blot of moth flight muscle protein separated by
SDS-PAGE and hybridized with MAC 145 antibody specific to insect Tnt. Relative
abundances of Tnt F transcripts from the same individuals (below each
lane) show agreement with the Tnt F protein isoforms. Specifically, these data
show that Tnt F was the predominant protein form when Tnt F
transcript relative abundance was high, and was about half of the protein when
Tnt F was about 50% of the transcript pool. (Adult moth photo
courtesy of Renn Tumlison.)
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