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Fig. 2 Recovery curves in FRAP experiments. Fluorescence intensities normalized by
the initial value just before photobleaching were plotted against time. The
data points and error bars indicate the mean ± s.d. For simplicity,
error bars are indicated only for every five points. Theoretical curves (solid
lines) obtained by fitting data to Eqn
1 are superimposed. Arrows indicate the time points of
photobleaching. (A–C) Recovery curves of flagella loaded with (A)
calcein, (B) carboxyfluorescein and (C) Oregon Green (N=6). (D) A
typical curve of FRAP in aqueous solution in the case where calcein in 10 mmol
l–1 HEPES (pH 7.0) and 20% glycerol (N=3) was used.
(E) Fluorescence recovery in heads (open squares) and concomitant fluorescence
decrease of whole flagella (open circles) when a photobleaching laser pulse
was directed to the head region (N=5). Note that the data are shown
with a longer time scale than for the other four.
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