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Fig. 2. 
23 N-truncated jShak1 channels lack N-type inactivation but exhibit
slow C-type inactivation. (A)The full-length wild-type jShak1 channel
expressed fast opening kinetics and fast N-type inactivation in
Xenopus oocytes. Outwardly directed current traces were evoked by 500
ms step depolarizations from a holding potential of –90 mV to a range of
potentials from –90 to +90 mV in 10 mV increments followed by a return
to –90 mV. (B)The 23 N-truncated wild-type jShak1
channel lacked N-type inactivation. Outwardly directed current traces were
evoked by 400 ms step depolarizations from a holding potential of –90 mV
to a range of potentials from –90 to +90 mV in 10 mV increments followed
by a return to the holding potential. (C)The IFR-E double mutant expressed
slow, C-type inactivation. Other N-truncated S2/S4 mutant jShak1 channels
showed lesser amounts of slow inactivation. Currents evoked as in B.
(D)Shortened acquisition protocols allowed channels to open fully but limited
the amount of slow inactivation. Outwardly directed currents from an IFR-E
mutant evoked by 50 ms step depolarizations from a holding potential of
–90 mV to a range of potentials from –90 to +90 mV in 10 mV
increments followed by a 20 ms step to –50 mV and 200 ms return to
holding potential.
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