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Fig. 6. (A) Occurrence of CO2 pulses from zero and non-zero baseline
CO2 in one specimen of Pterostichus stygicus. `Singlet'
pulses (indicated with grey arrows) were stand-alone bursts of CO2
that occurred with a tracheal compression. Such singlets did not occur in any
other specimen. The inset box shows the area (red) used to calculate the
volume of all other pulses. Beetle, mass 178.0 mg. (B) Multiple repeating
CO2 bursts used to simulate patterns of superimposition (without
additional CO2). A large singlet burst (red arrow in A) was
repeated at frequencies (f) of 14, 20, 33 and 50
min–1.