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Fig. 6. Effects of silencing AamOatp on Amblyomma americanum
fecundity. After feeding to repletion, ticks were incubated at 25°C for 20
days to lay eggs. Egg masses were weighed then divided by engorgement masses
(EMs) to calculate the egg mass conversion ratio (EMCR). The significance of
difference between the mean EMCRs of AamOatp-dsRNA (mean=0.423) and
control ticks non-injected (mean=0.541, P<0.0001) and those
injected with GFP-dsRNA (mean=0.515, P=0.0008) were determined using
the unpaired Student's t-test. The bars in this graph represent
maximum and minimum data points, as indicated in the text.