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Figure 3


Fig. 3. Temporal and spatial mRNA expression profile of AamOatp during tick feeding. (A) DNAse-treated total RNA of salivary gland (SG), midgut (MG), ovary (OV) and carcass (CA, tick remnants after removal of SG, MG and OV) dissected from ticks that were partially fed for 1–7 days were subjected to two-step semi-quantitative RT-PCR. (B) Densities of AamOatp and tick actin PCR bands were determined using the web-based ImageJ program, as described in the Materials and methods. Band densities representing mRNA levels in tick organs were normalized according to the formula Y=V+V(H–X)/X, where Y=normalized mRNA density, V=observed AamOatp PCR band density in individual tissues (MG, SG, OV and CA), H=highest tick actin PCR band density in tested tissues per time point, and X=tissue (MG, SG, OV and CA) tick actin PCR band density. The crosses, squares, triangles and circles indicate SG, MG, OV and CA, respectively.





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