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Figure 9


Fig. 9. (A) Effect of sequential addition of bafilomycin A1 (0.3 µmol l–1) and BAPTA-AM (25 µmol l–1) on the hypertonicity-induced changes in AO fluorescence. Data are means ± s.e.m. of 56–68 cells from three independent preparations. Data were normalized as in Fig. 3. (B) Changes in AO fluorescence following exposure of cells to A23187 (2 µmol l–1) in Ca2+-free medium followed by BAPTA-AM (25 µmol l–1), in the continuous presence of A23187 and absence of extracellular Ca2+. Data are means ± s.e.m. of 90 cells from five independent preparations. Data were normalized as in Fig. 3.





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