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Fig. 6. Changes in AO fluorescence following exposure of cells to
Na+-free medium or amiloride (100 µmol l–1)
under steady-state conditions followed by exposure to hypertonicity then
restoration of isotonic conditions, in the continuous absence of
Na+ or presence of amiloride. Data are means ± s.e.m. of
34–41 cells from three independent preparations. Data were normalized as
in Fig. 3. For comparison, the
control (Fig. 2A) is shown as a
dotted line.