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Fig. 4. Changes in AO fluorescence following exposure of cells to
Cl–-free medium or SITS (0.5 mmol l–1) under
steady-state conditions followed by exposure to hypertonicity then restoration
of isotonic conditions, in the continuous absence of Cl– or
presence of SITS. Data are means ± s.e.m. of 34–38 cells from
three independent preparations. Data were normalized as in
Fig. 3. For comparison, the
control (Fig. 2A) is shown as a
dotted line.