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Fig. 3. Changes in AO fluorescence following exposure of cells to valinomycin (10
µmol l–1) under steady-state conditions followed by
exposure to hypertonicity then restoration of isotonic conditions, in the
continuous presence of valinomycin. Data are means ± s.e.m. of 67 cells
from three independent preparations. Data were normalized to the mean
calculated from the first five points. For comparison, the control
(Fig. 2A) is shown as a dotted
line.