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Figure 2


Fig. 2. Changes in AO fluorescence in response to (A) exposure of cells to hypertonicity (1.6xisosmolarity) followed by restoration of isotonicity and (B) exposure of cells to bafilomycin A1 (0.3 µmol l–1) followed by the same exposure conditions as in A in the continuous presence of bafilomycin A1. Data are means ± s.e.m. of 64–66 cells from three to four independent preparations. Data were normalized to the mean calculated from five points preceding the hypertonicity exposure. Dotted line corresponds to normal quenching of AO (three independent preparations, 54 cells, data normalized to the mean calculated from the first five points).





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