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Figure 9


Fig. 9. The effect of 12 h exposure to 1% CO2 in air (CO2), 1% CO2 in air with 10 mmol l–1 Hepes buffered-water (CO2+Hepes), and 100% air with 10 mmol l–1 Hepes-buffered water (Air+Hepes) on the mRNA expression of carbonic anhydrase (CA2), H+-ATPase, NHE2 and Na+/K+-ATPase {alpha}-1a (NKA) in the gills of rainbow trout. Control fish were exposed to 100% air for 12 h in unbuffered water. Expression data were normalized to ng total RNA concentration with the control value set to one. Significant differences from the control are indicated by an asterisk (P<0.05). Data are means ± s.e.m. (N=6).





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