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Figure 6


Fig. 6. Gill Rh mRNA expression in rainbow trout after 12 h of exposure to 1% CO2 in air (CO2), 1% CO2 in air with 10 mmol l–1 Hepes-buffered water (CO2+Hepes), 100% air with 10 mmol l–1 Hepes-buffered water (Air+Hepes) and high environmental ammonia (HEA; 1.5 mmol NH4HCO3). Control fish were exposed to 100% air for 12 h in unbuffered water. Expression data were normalized to ng total RNA concentration with the control value set to one. Asterisks indicate significant difference from the control (P<0.05). Data are means ± s.e.m. (N=6).





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