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Fig. 4. The effect of 12 h exposure of rainbow trout to: hypercapnia (1%
CO2 in air) in unbuffered water, hypercapnia in 10 mmol
l–1 Hepes-buffered water, and normocapnia (100% air) in
Hepes-buffered water on the net ammonia flux (JAmm).
Control fish were exposed to normocapnia for 12 h in unbuffered water. The
negative values indicate excretion into the water. Crosses represent values
significantly different from the 3 h control value. Asterisks represent values
significantly different from the 12 h control value. Control values are
significantly different from each other (P<0.05). Data are means
± s.e.m. (N=6–10).