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Figure 1


Fig. 1. Epithelial Na+ channel (ENaC) currents revealed by single channel recording from cultured M1 cells. (A) In a cell-attached recording, inward currents were recorded when the pipette voltage (Vp) was held at +20 mV and +40 mV. (B) In an inside-out recording, the single channel currents were detected when the pipette voltage was held at +100 mV and +60 mV. (C) In an outside-out recording, the currents were monitored when the pipette voltage was held at –60 mV. Bath application of 5 µmol l–1 amiloride almost abolished the ENaC currents. The currents were reversed when amiloride was washed off. c indicates the closing level.





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