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Fig. 3 for affects recovery from acute food deprivation. (A) After one hour of food deprivation, sitter (for^s and for^s2) larvae increase their food intake to a greater extent than rover (for^R) larvae. After two and three hours of food deprivation, there were no significant differences in food intake between rovers and sitters. (B) Re-feeding experiments show that after three hours of acute food deprivation, followed by 0, 30 min, 1 h or 2 h of re-feeding, food intake levels in for^R larvae return to their initial well-fed food intake levels more slowly than in for^s and for^s2 larvae. (C) Hemolymph carbohydrate levels in for^R larvae returned to well-fed levels after 2 h of re-feeding, whereas hemolymph carbohydrate levels in for^s and for^s2 were not changed by acute food deprivation (see Fig. 2D) or re-feeding. (D) Well-fed for^R larvae have significantly lower akh mRNA levels than for^s and for^s2. Relative akh mRNA levels were measured using quantitative real-time PCR and standardized against for^R larvae. Error bars indicate means ± s.e.m.

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